human trf3 cdna Search Results


human trf3 cdna  (OriGene)
90
OriGene human trf3 cdna
Fig. 1 The <t>TRF3</t> expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values
Human Trf3 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+trf3+cdna/TBP2+(TBPL2)+(NM_199047)+Human+Tagged+ORF+Clone/pm32448362-71-7-12
Average 90 stars, based on 1 article reviews
human trf3 cdna - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
tbph cdna  (OriGene)
90
OriGene tbph cdna
Fig. 1 The <t>TRF3</t> expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values
Tbph Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+trf3+cdna/TBP2+(TBPL2)+(NM_199047)+Human+Untagged+Clone/pmc02890254-120-0-5
Average 90 stars, based on 1 article reviews
tbph cdna - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

Journal: Stem cell research & therapy

Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

doi: 10.1186/s13287-020-01711-w

Figure Lengend Snippet: Fig. 1 The TRF3 expression level is enhanced during ME differentiation of hESCs. a Microscope images for the morphology of ME differentiation. Scale bar = 200 μm. b The heat map of the expression pattern of early germ layer genes during the ME differentiation of hESCs based on the microarray analysis. The expression values in log2 scale were calculated and presented on the heat map with red representing highly abundant transcripts and green representing poorly abundant transcripts. n = 3 each. c qRT-PCR analysis of TBP, TRF2, and TRF3. Data are presented as mean ± SEM. n = 3 each. *p < 0.05, **p < 0.01 compared with the corresponding undifferentiated values

Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

Techniques: Expressing, Microscopy, Microarray, Quantitative RT-PCR

Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

Journal: Stem cell research & therapy

Article Title: TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.

doi: 10.1186/s13287-020-01711-w

Figure Lengend Snippet: Fig. 3 The expression levels of pluripotent markers are comparable among the undifferentiated TRF3+/+, TRF3−/−-1, and TRF3−/−-2 hESCs. a qRT- PCR analysis of pluripotency markers OCT4 and NANOG. n = 3. b Flow cytometry analysis of OCT4 and SSEA4. c Immunocytochemical staining analysis of OCT4 and SSEA4. Similar results were obtained from three independent experiments. Scale bar = 50 μm. d Flow cytometry analysis of cell cycle in undifferentiated hESCs. n = 3. e Representative analysis of clones positive for alkaline phosphatase staining. Similar results were obtained from three independent experiments. Scale bar = 500 μm. Data are presented as mean ± SEM

Article Snippet: Reintroduction of TRF3 into TRF3−/− hESCs The human TRF3 cDNA (RC211988, NM_199047, OriGene, USA) was cloned into pCDH-EF1-3×Flag-MCST2A-Puro, modified from pCDH-EF1-MCS-T2A-Puro (System Biosciences, CA, USA), and named as pCDHEF1-3×Flag-TRF3-T2A-Puro.

Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, Staining, Clone Assay